Journal: Poultry Science
Article Title: Co-infection with Proteus mirabilis and extraintestinal pathogenic Escherichia coli aggravated liver injury in chickens by disrupting the jejunal barrier
doi: 10.1016/j.psj.2025.105369
Figure Lengend Snippet: Proteus mirabilis disrupts the jejunal barrier to enhance co-infection via inflammation.a. Comparison of villus length and crypt depth in the jejunum stained with H&E (scale = 100 μm); b. Proportion of goblet cells on villus in the jejunum stained with Alcian Blue-PAS (scale = 25 μm); c. Mucin 2 secretion in the jejunum by immunofluorescence staining (blue: DAPI; red: Mucin 2); d. ZO-1 secretion in the jejunum by immunofluorescence staining (blue: DAPI; red: ZO-1); e. mRNA levels of IL-1β, IL-18, NF-κB , and TNF-α in the jejunum. Statistical significance: For ANOVA, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001.
Article Snippet: Additionally, jejunum paraffin sections (5 μm, n = 3) were incubated overnight at 4°C with primary antibodies against MUC2 (GB11344, Servicebio, China) and ZO-1( GB111402 , Servicebio, China).
Techniques: Infection, Comparison, Staining, Immunofluorescence